Source code for

"""Experimental time kernel module."""
from typing import Any, Dict, Tuple, Mapping, Optional

from abc import ABC
from copy import copy
from types import MappingProxyType

import scanpy as sc
from import ModeEnum
from cellrank.ul._docs import d
from import _normalize
from import Kernel
from import _ensure_numeric_ordered
from import AnnData

import numpy as np
import pandas as pd
from scipy.sparse import bmat, spdiags

from matplotlib.colors import Normalize, to_hex
from matplotlib.pyplot import get_cmap

class LastTimePoint(ModeEnum):  # noqa
    UNIFORM = "uniform"
    DIAGONAL = "diagonal"
    CONNECTIVITIES = "connectivities"

[docs]@d.dedent class ExperimentalTimeKernel(Kernel, ABC): """ Kernel base class which computes directed transition probabilities based on experimental time. %(density_correction)s Parameters ---------- %(adata)s %(backward)s time_key Key in :attr:`adata` ``.obs`` where experimental time is stored. The experimental time can be of either of a numeric or an ordered categorical type. %(cond_num)s """ def __init__( self, adata: AnnData, backward: bool = False, time_key: str = "exp_time", compute_cond_num: bool = False, **kwargs: Any, ): super().__init__( adata, backward=backward, time_key=time_key, compute_cond_num=compute_cond_num, check_connectivity=False, **kwargs, ) self._time_key = time_key def _read_from_adata(self, **kwargs: Any) -> None: super()._read_from_adata(**kwargs) time_key = kwargs.pop("time_key", "exp_time") self._exp_time = _ensure_numeric_ordered(self.adata, time_key) self.adata.obs[time_key] = self.experimental_time.values # fmt: off cmap = get_cmap(kwargs.pop("cmap", "gnuplot")) cats = norm = Normalize(vmin=cats.min(), vmax=cats.max()) self.adata.uns[f"{time_key}_colors"] = np.array([to_hex(c) for c in cmap(norm(cats) * cmap.N)]) # fmt: on
[docs] @d.dedent def plot_single_flow( self, cluster: str, cluster_key: str, time_key: Optional[str] = None, *args: Any, **kwargs: Any, ) -> None: """ %(plot_single_flow.full_desc)s Parameters ---------- %(plot_single_flow.parameters)s Returns ------- %(plot_single_flow.returns)s """ # noqa: D400 if time_key is None: time_key = self._time_key return super().plot_single_flow(cluster, cluster_key, time_key, *args, **kwargs)
@property def experimental_time(self) -> pd.Series: """Experimental time.""" return self._exp_time
[docs] def copy(self) -> "ExperimentalTimeKernel": """Return a copy of self.""" pk = type(self)(self.adata, backward=self.backward, time_key=self._time_key) pk._exp_time = copy(self.experimental_time) pk._params = copy(self._params) pk._cond_num = self.condition_number pk._transition_matrix = copy(self._transition_matrix) return pk
def __invert__(self) -> "ExperimentalTimeKernel": super().__invert__() if len( > 1: minn, maxx = self.experimental_time.min(), self.experimental_time.max() self._exp_time = pd.Series( pd.Categorical( maxx - np.array(self.experimental_time) + minn, ordered=True ), index=self.experimental_time.index, ) return self
[docs]class TransportMapKernel(ExperimentalTimeKernel, ABC): """Kernel base class which computes transition matrix based on transport maps for consecutive time pairs.""" def __init__(self, *args: Any, **kwargs: Any): super().__init__(*args, **kwargs) self._tmaps = None def _restich_tmaps( self, tmaps: Mapping[Tuple[float, float], AnnData], last_time_point: LastTimePoint = LastTimePoint.DIAGONAL, conn_kwargs: Mapping[str, Any] = MappingProxyType({}), normalize: bool = True, ) -> AnnData: from import ConnectivityKernel conn_kwargs = dict(conn_kwargs) conn_kwargs["copy"] = False _ = conn_kwargs.pop("key_added", None) density_normalize = conn_kwargs.pop("density_normalize", True) blocks = [[None] * (len(tmaps) + 1) for _ in range(len(tmaps) + 1)] nrows, ncols = 0, 0 obs_names, obs = [], [] for i, tmap in enumerate(tmaps.values()): blocks[i][i + 1] = _normalize(tmap.X) if normalize else tmap.X nrows += tmap.n_obs ncols += tmap.n_vars obs_names.extend(tmap.obs_names) obs.append(tmap.obs) obs_names.extend(tmap.var_names) n = self.adata.n_obs - nrows if last_time_point == LastTimePoint.DIAGONAL: blocks[-1][-1] = spdiags([1] * n, 0, n, n) elif last_time_point == LastTimePoint.UNIFORM: blocks[-1][-1] = np.ones((n, n)) / float(n) elif last_time_point == LastTimePoint.CONNECTIVITIES: adata_subset = self.adata[tmap.var_names].copy() sc.pp.neighbors(adata_subset, **conn_kwargs) blocks[-1][-1] = ( ConnectivityKernel(adata_subset) .compute_transition_matrix(density_normalize) .transition_matrix ) else: raise NotImplementedError( f"Last time point mode `{last_time_point}` is not yet implemented." ) # prevent the last block from disappearing n = blocks[0][1].shape[0] blocks[0][0] = spdiags([], 0, n, n) tmp = AnnData(bmat(blocks, format="csr")) tmp.obs_names = obs_names tmp.var_names = obs_names tmp = tmp[self.adata.obs_names, :][:, self.adata.obs_names] tmp.obs = pd.merge( tmp.obs, pd.concat(obs), left_index=True, right_index=True, how="left", ) return tmp @property def transport_maps(self) -> Optional[Dict[Tuple[float, float], AnnData]]: """Transport maps for consecutive time pairs.""" return self._tmaps